Journal: Advanced Science

Article Title: Acid‐Responsive Dual‐Targeted Nanoparticles Encapsulated Aspirin Rescue the Immune Activation and Phenotype in Autism Spectrum Disorder

doi: 10.1002/advs.202104286

Figure Lengend Snippet: Immunostaining of CD11b marker to show the microglia activation in in vivo condition. A) The microglia cell immunostaining of CD11b marker was shown in the slide. B) PolyI/C induced increased staining in CD11b. C) Asp@TMNPs inhibited CD11b staining compared with PolyI/C treatment group. The down‐panel figure is the enlarged picture of up‐panel.

Article Snippet: Brain slides were operated by immunofluorescence of anti‐CD11b (bs‐1014R‐AF488, Bioss) antibody and others were stained with DAPI.

Techniques: Immunostaining, Marker, Activation Assay, In Vivo, Staining

Journal: Molecular Metabolism

Article Title: Loss of Tbk1 kinase activity protects mice from diet-induced metabolic dysfunction

doi: 10.1016/j.molmet.2018.06.007

Figure Lengend Snippet: Tbk1 Δ/ Δ mice have less inflammation relative to Tbk1 +/ + mice on HFD . (A) Representative 20x images and quantification of cd11b/c stained subcutaneous white adipose tissue from Tbk1 Δ/Δ and Tbk1 +/+ mice after 10 weeks on ND or HFD (n = 4–8 mice/group). Scale bar indicates 200 μM and ‘CLS’ refers to crown like structures. (B) mRNA expression of genes encoding CD11c, F4/80, TNFα, IL-6 and IL-12 in subcutaneous WAT of Tbk1 Δ/Δ and Tbk1 +/+ mice fed with HFD as indicated (n = 4–6 mice/group). (C) Liver tissue lysates from 14-week-old HFD-fed Tbk1 Δ/Δ and Tbk1 +/+ mice were immunoblotted with antibodies against IL-6 and IL-1β. β-actin and GAPDH were used as internal loading controls. Indicated cytokines from WAT (D) and liver tissue (E) lysates of HFD-fed Tbk1 +/+ and Tbk1 Δ/Δ mice were measured by Bio-Rad multiplex array (n = 4–8 mice/group for WAT, n = 8–12 mice/group for liver). All mice are from 129S5 background. Results are representative of mean +/− SEM. Statistical analysis by Student's t -test. * p < 0.05, ** p < 0.01.

Article Snippet: Sections for immunohistochemical analysis were blocked with 20% aquablock and incubated with rabbit anti-cd11b/c (Novus Biologicals, NB110-40766) in blocking solution (5% BSA in TBS with 0.05% tween) at 4 °C overnight.

Techniques: Staining, Expressing, Multiplex Assay

Journal: Journal of Neuroinflammation

Article Title: LPA 1 signaling drives Schwann cell dedifferentiation in experimental autoimmune neuritis

doi: 10.1186/s12974-021-02350-5

Figure Lengend Snippet: Minor impact of AM095 treatment on immune cell infiltration. Immune cell infiltration was assessed by counting the number of CD3 + (T-lymphocytes) and CD11b/c + (myeloid cells) on sciatic nerve sections in randomly selected fields. a Representative images of immune cell infiltration at peak of disease at 14 dpi, and early remission phase at 21 dpi. b + c No significant difference was observed in the number of infiltrating CD3+ cells between peak of disease and remission phase ( N = 9/8/4/4 from left to right). d + e While there was no significant reduction in CD3 + cells at 14 dpi, we recognized a slight decrease in myeloid cells under AM095 at 21 dpi ( N = 5/5/5/6 from left to right)). Cell counts were normalized to area. Data represent mean ± s.e.m. P ≤ 0.05*. Scale bars indicate 50 µm

Article Snippet: The following antibodies were used at the indicated dilutions: rabbit monoclonal [SP7] anti-CD3 (abcam; diluted 1:500); mouse monoclonal [1F4] anti-CD3 (BioLegend; diluted 1:1000); mouse monoclonal [OX42] anti-CD11b/c (abcam; diluted 1:1000); rabbit polyclonal anti-Sox2 IgG (abcam, ab97959; diluted 1:200); rabbit monoclonal [EPR4007] anti-Sox10 (abcam; diluted 1:250).

Techniques: